Characterisation of the Putative Antigenic Genes of the Outer Membrane Proteins of Pasteurella multocida B:2 Strain PMTB2.1 through in silico Analysis

Outer membrane proteins (OMPs), usually found in Gram-negative bacteria, have long been shown to elicit immune responses infected hosts. This tendency of OMPs generate reactions makes them ideal candidates for vaccine development against pathogenic bacteria. Pasteurella multocida is a pathogen responsible the economically significant veterinary disease, hemorrhagic septicemia (HS). HS an endemic and highly fatal disease affecting buffaloes cattle. In Malaysia, outbreaks this cost about half million USD each year. Thus, despite current treatment prevention measures, prevalent issue that needs be overcome. subsp. PMTB2.1, Malaysian strain pathogen, has recently had its entire genome sequenced after being isolated from region. Antigenic since identified published further characterisation. LptD, Wza, TbpA are integral proteins, while Pal peripheral protein not characterised in-depth. study, therefore, aims analyse these through silico methods. First, homology modelling was performed using SWISS-MODEL, whereafter, structures generated were validated SWISS-MODEL structure assessment page, PROCHECK, ERRAT, PROSA programs. The Pal, good models, LptD near-good model based on validation performed. Analyses BCPREDS, NetMHCpan4.1, NetBoLAIIpan1.0 revealed four could potentially humoral cellular responses.